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Advantages And Disadvantages Of Electroforesis

Advantages And Disadvantages Of Electroforesis

Electroforesis is the process of moving charged molecules in an electric field. The speed of molecules moving in the electric field depends on the charge, shape and size. The position of molecules that are stranded on the gel can be detected by colouring or autoradiography, or even quantification with a densitometer. So what are the advantages and disadvantages of this electroforesis? Check out the following explanation.

Advantages Of electroforesis

– Flexibility in identification
Electroforesis is a versatile diagnostic test that can be used for the separation of proteins and nucleic acids. This system is often used in the medical field to diagnose various blood genetic disorders such as anaemia and thalassemia or blood disorders. Electroforesis has also been used to determine various fish species and to determine the valuable properties of soy and wheat by analyzing and separating DNA components.

– Accuracy of results
Electroforesis is very accurate. When the process is done correctly, this can separate the proteins present in the cell into as many as 1,500 different parts. This system is also so selective that it can see differences in DNA samples, even if the sample is different from two pairs of bases. It provides reliable test results that enable scientists and laboratory technicians to draw conclusions about the genetic hardier plant species, determine the possibility of disease in the human body and maybe one day eliminate it at the genetic level.

Disadvantages Of Electroforesis

– Electrophoretic measurements are incorrect
Electroforesis gel can effectively separate similar proteins of different weights. This can separate them more precisely through a technique known as 2D electroforesis. This is common in proteomics.

To get the right mass (weight) of proteins, spectroscopy must be used after the protein has been purified by electroforesis. Besides, comparing the relative number of different molecules depends on the density of bands (darkness) of different places on the gel. This method has several levels of error and the sample is usually run several times to get clean results.

– Only certain molecules can be visualized
Electroforesis is very good at separating and identifying biomolecules from the middle to large levels. However, many of the molecules that researchers hope to see are smaller such as hormones, neurotransmitters and ions that are not measured by electroforesis. This is for two reasons: They do not properly react with electroforesis preparations and even if they do, they are too small to separate properly and will rush out at the bottom of the gel. These molecules are not measured by techniques such as RIAAs (radioimmunoassays) and ELISAs (enzyme-linked immunosorbent assay).

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